The transition from oocyte-coded to embryo-coded glucose phosphate isomerase in the early mouse embryo.
نویسندگان
چکیده
The proportions of glucose phosphate isomerase (GPI-1) allozymes produced by early (Gpi-1sa/Gpi-1sb female X Gpi-1sc/Gpi-1sc male)F1 mouse embryos were analysed by quantitative cellulose acetate electrophoresis. Technical controls showed that this system is extremely sensitive, quantitatively reproducible and quite accurate. Genetic controls established that the Gpi-1sa/Gpi-1sb mothers were homozygous for the Gpi-1tb temporal allele, that produces relatively high GPI-1 activity in the oocyte. The oocyte-coded enzyme lasted until about 5 1/2 days post coitum (p.c.) or shortly thereafter. The maternally derived, embryonic Gpi-1s allele was expressed no earlier than the paternally derived allele. This was first expressed between 2 1/2 and 3 1/2 days p.c. In this cross, most of the transition from oocyte-coded to embryo-coded GPI-1 occurred between 2 1/2 and 5 1/2 days p.c.
منابع مشابه
Genetic differences in glucose phosphate isomerase activity among mouse embryos.
We have compared mouse embryos of three heterozygous, congenic genotypes (with high, medium and low levels of oocyte-coded glucose phosphate isomerase (GPI-1) activity respectively) to test whether 1) the survival time of oocyte-coded GPI-1 activity in the early embryo is affected by its activity level in the oocyte and 2) whether embryo-coded GPI-1 is detected earlier in embryos that inherit l...
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ورودعنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 78 شماره
صفحات -
تاریخ انتشار 1983